General techniques
- Procedure for incoming plasmids
- Preparation of glycerol stocks
- Ethanol precipitation of DNA or RNA
- Phenol-Chloroform extraction of nucleic acids
- Cleavage of DNA into mononucleotides
Plasmid DNA isolation
- Minipreps using Promega kit (also available in french)
- Midipreps using Macherey & Nagel Kit
- BAC miniprep
Plasmid manipulation
- Ligation (also available in french): excel sheet for calculations
- Gibson assembly: excel sheet for calculations
- Annealing and ligation of linkers
- Recombination of BAC with PCR product
- Restriction enzyme digestion (also in french with agarose gel elecrophoresis)
- Dephosphorylation of plasmids
Transformation and preparation of competent cells
- Preparation of electrocompetent cells
- Electroporation of electrocompetent cells
- Rapid preparation of electrocompetent cells
- Preparation of chemically competent cells (also in french)
- Transformation into chemically competent cells
Extraction of DNA from mammalian cells:
- genomic DNA extraction
- extrachromosomal DNA