• Home
  • Research
      • Back
      • Metabolite repair
      • Cancer Metabolism
      • Ribitolphosphorylation
      • Neurodegeneration
      • Inherited errors of metabolism
      • Metabolism of 'weird' lipids
      • miRNAs
  • Team
      • Back
      • Guido Bommer
      • Maria Veiga-Da-Cunha
      • Emile Van Schaftingen
      • Marina Bury
      • Francesco Caligiore
      • Nathalie Chevalier
      • Joseph Dewulf
      • Isabelle Gerin
      • Jean Jacobs
      • Julie Graff
      • Isaac Heremans
      • Takfarinas Kentache
      • Stephanie Paquay
      • Alumni
  • Protocols
      • Back
      • DNA methods
      • RNA methods
      • Protein methods
      • Cell Culture methods
      • Biochemical methods
      • Inventory
  • The Lab
  • Publications
  • Equipment
      • Back
      • GC-MS signup
      • LC-MS signup
      • HPLC-signup
      • Gel and WB imaging station
  • Contact

DNA methods

General techniques

  • Procedure for incoming plasmids
  • Preparation of glycerol stocks
  • Ethanol precipitation of DNA or RNA
  • Phenol-Chloroform extraction of nucleic acids
  • Cleavage of DNA into mononucleotides

Plasmid DNA isolation

  • Minipreps using Promega kit (also available in french)
  • Midipreps using Macherey & Nagel Kit
  • BAC miniprep

Plasmid manipulation

  • Ligation (also available in french): excel sheet for calculations
  • Gibson assembly: excel sheet for calculations
  • Annealing and ligation of linkers
  • Recombination of BAC with PCR product
  • Restriction enzyme digestion (also in french with agarose gel elecrophoresis)
  • Dephosphorylation of plasmids

Transformation and preparation of competent cells

  • Preparation of electrocompetent cells
  • Electroporation of electrocompetent cells
  • Rapid preparation of electrocompetent cells
  • Preparation of chemically competent cells  (also in french)
  • Transformation into chemically competent cells

Extraction of DNA from mammalian cells:

  • genomic DNA extraction
    • 12-well plate
    • 96-well plate
    • mouse tail
    • Isolation of DNA from TRIZOL
  • extrachromosomal DNA
    •  


 

 

 

 

© 2016 by Guido Bommer.