General maintenance
- general rules for the tissue culture room (english) and techniques (french)
- mycoplasma PCR (english) (french)
Transfection and infections
- Production of recombinant Lentiviruses (excel sheet with calculations)
- Production of recombinant Retroviruses
- Transfection with siRNA and plasmid together
- Preparation of linear polyethyleneimine (PEI)
- Transfection with PEI
- Production of recombinant adeno-associated viruses
- Culture of SF9 cells
- Production and expansion of recombinant Baculovirus
- Determining titers for baculovirus
Specialized culture conditions and growth assessment
- Culture and differentiation of 3T3L1 cells
- soft agar assay
- Delipidating serum
- Embryoid body protocol (french)
- Alamar Blue assay with Rezazurin
- growth curve with crystal violet
- MTT-test
Metabolic assessment
- Oxygen consumption and extracellular acidification (Seahorse)
- H2-DCFDA assay to analyze H2O2 by flow cytometry
- Glucose consumption with tritiated glucose
Imaging:
- Staining cells for immunofluorescence
- Cleaning coverslips before immunofluorescence
- MOWIOL embedding medium
- HE staining
- Pappenheim staining
- Oil Red O staining
Flow cytometry
- Annexin V and PI staining to determine apoptosis
- Double thymidine block
- Thymidine-Nocodazole block
- EdU assay to assess cells in S-phase
- Usage of the FACSverse flow cytometer
- Usage of the Fortessa flow cytometer
- Usage of FACScalibur