General maintenance

• general rules for the tissue culture room (english) and techniques (french)
• mycoplasma PCR (english) (french) 

Transfection and infections

• Production of recombinant Lentiviruses (excel sheet with calculations)
• Production of recombinant Retroviruses
• Transfection with siRNA and plasmid together
• Preparation of linear polyethyleneimine (PEI)
• Transfection with PEI
• Production of recombinant adeno-associated viruses
• Culture of SF9 cells
• Production and expansion of recombinant Baculovirus
• Determining titers for baculovirus

Specialized culture conditions and growth assessment

• Culture and differentiation of 3T3L1 cells
• soft agar assay
• Delipidating serum
• Embryoid body protocol (french)
• Alamar Blue assay with Rezazurin
• growth curve with crystal violet
• MTT-test

Metabolic assessment

• Oxygen consumption and extracellular acidification (Seahorse)
• H2-DCFDA assay to analyze H2O2 by flow cytometry
• Glucose consumption with tritiated glucose

Imaging:

• Staining cells for immunofluorescence
• Cleaning coverslips before immunofluorescence
• MOWIOL embedding medium
• HE staining
• Pappenheim staining
• Oil Red O staining

Flow cytometry

• Annexin V and PI staining to determine apoptosis
• Double thymidine block
• Thymidine-Nocodazole block
• EdU assay to assess cells in S-phase
• Usage of the FACSverse flow cytometer
• Usage of the Fortessa flow cytometer
• Usage of FACScalibur